human immortalized epidermal cell line hela Search Results


immortalized non-tumorigenic human epidermal cell line hacat  (Shiseido Inc)
90
Shiseido Inc immortalized non-tumorigenic human epidermal cell line hacat
BITC decreases Mis12 protein expression in a proteasome-dependent manner. The Mtw1/Mis12 protein level was determined by a Western blot analysis. Total protein staining or actin was used as the loading control. ( A ) BY4741 cells expressing the Mtw1-TAP were treated with BITC for 24 h and Mtw1-TAP protein level was determined. ( B ) HCT-116 cells were treated with BITC for 24 h and the Mis12 protein level was determined. ( C ) <t>HaCaT</t> cells were treated with BITC for 24 h and the Mis12 protein level was determined. ( D ) HCT-116 cells were treated with 20 μM BITC for the indicated hours and the Mis12 protein level was determined. ( E ) HCT-116 cells stably expressing HA-Mis12 were treated with 20 μM BITC for 1 h. The signals of HA-Mis12 (green) and DNA (blue) were detected by immunofluorescence. The signal intensity of HA-Mis12 at each kinetochore was measured relative to an adjacent background signal. Bar, 10 µm. Fluorescence intensity was quantified using Image J software. ( F ) HCT-116 cells were treated with 20 μM BITC for 1 h. The signals of the microtubules (green) and DNA (blue) were detected by immunofluorescence. Bar, 10 µm. ( G ) HCT-116 cells were treated with BITC for 24 h and the Mis12 mRNA level was determined by RT-PCR. ( H ) HCT-116 cells were treated with 10 μM MG132 and/or 20 μM BITC for 24 h, and the Mis12 protein level was determined. ( I ) HCT-116 cells stably expressing HA-Mis12 were treated with 10 μM MG132 and/or 20 μM BITC for 30 min. The whole cell lysates were immunoprecipitated with the HA antibody and visualized by a Western blot analysis with ubiquitin, phosphoserine/threonine/tyrosine or HA-tag antibody. ( J ) HCT-116 cells were treated with 100 nM paclitaxel for 24 h and subjected to Western blot analysis to detect Mis12 signal. Actin was used as a loading control. ( K ) HCT-116 cells were treated with 100 nM paclitaxel for 24 h and subjected to MTT assay to measure cell viability. The values represent means ± SEM of three separate experiments (* P < 0.05 compared with control; Student’s t -test).
Immortalized Non Tumorigenic Human Epidermal Cell Line Hacat, supplied by Shiseido Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human immortalized epidermal cell line hacat  (Corning Life Sciences)
90
Corning Life Sciences human immortalized epidermal cell line hacat
BITC decreases Mis12 protein expression in a proteasome-dependent manner. The Mtw1/Mis12 protein level was determined by a Western blot analysis. Total protein staining or actin was used as the loading control. ( A ) BY4741 cells expressing the Mtw1-TAP were treated with BITC for 24 h and Mtw1-TAP protein level was determined. ( B ) HCT-116 cells were treated with BITC for 24 h and the Mis12 protein level was determined. ( C ) <t>HaCaT</t> cells were treated with BITC for 24 h and the Mis12 protein level was determined. ( D ) HCT-116 cells were treated with 20 μM BITC for the indicated hours and the Mis12 protein level was determined. ( E ) HCT-116 cells stably expressing HA-Mis12 were treated with 20 μM BITC for 1 h. The signals of HA-Mis12 (green) and DNA (blue) were detected by immunofluorescence. The signal intensity of HA-Mis12 at each kinetochore was measured relative to an adjacent background signal. Bar, 10 µm. Fluorescence intensity was quantified using Image J software. ( F ) HCT-116 cells were treated with 20 μM BITC for 1 h. The signals of the microtubules (green) and DNA (blue) were detected by immunofluorescence. Bar, 10 µm. ( G ) HCT-116 cells were treated with BITC for 24 h and the Mis12 mRNA level was determined by RT-PCR. ( H ) HCT-116 cells were treated with 10 μM MG132 and/or 20 μM BITC for 24 h, and the Mis12 protein level was determined. ( I ) HCT-116 cells stably expressing HA-Mis12 were treated with 10 μM MG132 and/or 20 μM BITC for 30 min. The whole cell lysates were immunoprecipitated with the HA antibody and visualized by a Western blot analysis with ubiquitin, phosphoserine/threonine/tyrosine or HA-tag antibody. ( J ) HCT-116 cells were treated with 100 nM paclitaxel for 24 h and subjected to Western blot analysis to detect Mis12 signal. Actin was used as a loading control. ( K ) HCT-116 cells were treated with 100 nM paclitaxel for 24 h and subjected to MTT assay to measure cell viability. The values represent means ± SEM of three separate experiments (* P < 0.05 compared with control; Student’s t -test).
Human Immortalized Epidermal Cell Line Hacat, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human immortalized epidermal cell line hacat/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
human immortalized epidermal cell line hacat - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

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BITC decreases Mis12 protein expression in a proteasome-dependent manner. The Mtw1/Mis12 protein level was determined by a Western blot analysis. Total protein staining or actin was used as the loading control. ( A ) BY4741 cells expressing the Mtw1-TAP were treated with BITC for 24 h and Mtw1-TAP protein level was determined. ( B ) HCT-116 cells were treated with BITC for 24 h and the Mis12 protein level was determined. ( C ) HaCaT cells were treated with BITC for 24 h and the Mis12 protein level was determined. ( D ) HCT-116 cells were treated with 20 μM BITC for the indicated hours and the Mis12 protein level was determined. ( E ) HCT-116 cells stably expressing HA-Mis12 were treated with 20 μM BITC for 1 h. The signals of HA-Mis12 (green) and DNA (blue) were detected by immunofluorescence. The signal intensity of HA-Mis12 at each kinetochore was measured relative to an adjacent background signal. Bar, 10 µm. Fluorescence intensity was quantified using Image J software. ( F ) HCT-116 cells were treated with 20 μM BITC for 1 h. The signals of the microtubules (green) and DNA (blue) were detected by immunofluorescence. Bar, 10 µm. ( G ) HCT-116 cells were treated with BITC for 24 h and the Mis12 mRNA level was determined by RT-PCR. ( H ) HCT-116 cells were treated with 10 μM MG132 and/or 20 μM BITC for 24 h, and the Mis12 protein level was determined. ( I ) HCT-116 cells stably expressing HA-Mis12 were treated with 10 μM MG132 and/or 20 μM BITC for 30 min. The whole cell lysates were immunoprecipitated with the HA antibody and visualized by a Western blot analysis with ubiquitin, phosphoserine/threonine/tyrosine or HA-tag antibody. ( J ) HCT-116 cells were treated with 100 nM paclitaxel for 24 h and subjected to Western blot analysis to detect Mis12 signal. Actin was used as a loading control. ( K ) HCT-116 cells were treated with 100 nM paclitaxel for 24 h and subjected to MTT assay to measure cell viability. The values represent means ± SEM of three separate experiments (* P < 0.05 compared with control; Student’s t -test).

Journal: Scientific Reports

Article Title: Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12

doi: 10.1038/s41598-019-45248-2

Figure Lengend Snippet: BITC decreases Mis12 protein expression in a proteasome-dependent manner. The Mtw1/Mis12 protein level was determined by a Western blot analysis. Total protein staining or actin was used as the loading control. ( A ) BY4741 cells expressing the Mtw1-TAP were treated with BITC for 24 h and Mtw1-TAP protein level was determined. ( B ) HCT-116 cells were treated with BITC for 24 h and the Mis12 protein level was determined. ( C ) HaCaT cells were treated with BITC for 24 h and the Mis12 protein level was determined. ( D ) HCT-116 cells were treated with 20 μM BITC for the indicated hours and the Mis12 protein level was determined. ( E ) HCT-116 cells stably expressing HA-Mis12 were treated with 20 μM BITC for 1 h. The signals of HA-Mis12 (green) and DNA (blue) were detected by immunofluorescence. The signal intensity of HA-Mis12 at each kinetochore was measured relative to an adjacent background signal. Bar, 10 µm. Fluorescence intensity was quantified using Image J software. ( F ) HCT-116 cells were treated with 20 μM BITC for 1 h. The signals of the microtubules (green) and DNA (blue) were detected by immunofluorescence. Bar, 10 µm. ( G ) HCT-116 cells were treated with BITC for 24 h and the Mis12 mRNA level was determined by RT-PCR. ( H ) HCT-116 cells were treated with 10 μM MG132 and/or 20 μM BITC for 24 h, and the Mis12 protein level was determined. ( I ) HCT-116 cells stably expressing HA-Mis12 were treated with 10 μM MG132 and/or 20 μM BITC for 30 min. The whole cell lysates were immunoprecipitated with the HA antibody and visualized by a Western blot analysis with ubiquitin, phosphoserine/threonine/tyrosine or HA-tag antibody. ( J ) HCT-116 cells were treated with 100 nM paclitaxel for 24 h and subjected to Western blot analysis to detect Mis12 signal. Actin was used as a loading control. ( K ) HCT-116 cells were treated with 100 nM paclitaxel for 24 h and subjected to MTT assay to measure cell viability. The values represent means ± SEM of three separate experiments (* P < 0.05 compared with control; Student’s t -test).

Article Snippet: Immortalized non-tumorigenic human epidermal cell line HaCaT was kindly provided by Shiseido Research Center (Yokohama, Japan).

Techniques: Expressing, Western Blot, Staining, Control, Stable Transfection, Immunofluorescence, Fluorescence, Software, Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation, Ubiquitin Proteomics, MTT Assay